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biotin pna  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotin pna
    Biotin Pna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 919 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1075-5/Biotinylated+Peanut+Agglutinin+(PNA)/pm42878060-206-20-21
    Average 95 stars, based on 919 article reviews
    biotin pna - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Control:

    Article Title: Concanavalin A targets phylogenetically conserved N-linked glycans on coronavirus spike proteins for broad-spectrum antiviral activity.
    Article Snippet: Concanavalin A used in this study was commercially purchased from Sigma-Aldrich (C0412-5MG) and was dissolved in PBS. .. Control lectins such as peanut agglutinin (PNA) (B-1075-5) and wheat germ agglutinin (WGA) (RL-1022-5) were purchased from Vector Laboratory. .. Recombinant glycosidase Endo H (P0702L) and PNGase F (P0704L) were purchased from New England Biolabs and were used according to the manufacturer’s instructions prior to pulldown assays.

    Whole Genome Amplification:

    Article Title: Concanavalin A targets phylogenetically conserved N-linked glycans on coronavirus spike proteins for broad-spectrum antiviral activity.
    Article Snippet: Concanavalin A used in this study was commercially purchased from Sigma-Aldrich (C0412-5MG) and was dissolved in PBS. .. Control lectins such as peanut agglutinin (PNA) (B-1075-5) and wheat germ agglutinin (WGA) (RL-1022-5) were purchased from Vector Laboratory. .. Recombinant glycosidase Endo H (P0702L) and PNGase F (P0704L) were purchased from New England Biolabs and were used according to the manufacturer’s instructions prior to pulldown assays.

    Article Title: Mucin Biology as a Local Diagnostic and Promising Therapeutic Target in Endometriosis: Expression and Glycosylation Profiling.
    Article Snippet: The primary antibodies used in this study included mouse anti-MUC1/EMA (clone E29, M0613, Agilent Technologies, Santa Clara, CA, USA), rabbit anti-MUC2 (ab134119, Abcam), mouse anti-MUC6 (sc-33668, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-MUC16/CA 125 (clone 5E1, MABC1608, Sigma-Aldrich, St. Louis, MO, USA), and mouse anti-MUC5AC (clone 45M1, MA5-12178, Thermo Fisher Scientific, Waltham, MA, USA) and rabbit antiserum MAN-5ACI for the polypeptides of MUC5AC (generous gift from Prof. Dr. David Thornton) [45]. .. In addition, several lectins were employed to detect specific glycan structures, including TRITC-labelled Dolichos biflorus agglutinin (DBA, GlycoMatrixTM, Dublin, OH, USA), biotinylated Maackia amurensis lectin II (MAL II), biotinylated Sambucus nigra lectin (SNA), FITC-labelled wheat germ agglutinin (WGA, Sigma Aldrich), biotinylated peanut agglutinin (PNA), FITC-labelled Aleuria aurantia lectin (AAL), and FITC-labelled Ulex europaeus agglutinin I (UEA I), all obtained from Vector Laboratories, Inc. (Newark, NJ, USA). ..

    Incubation:

    Article Title: Galectin-like protein 2: A new non-mammalian member of the galectin family.
    Article Snippet: .. The slides were then incubated with the biotinylated lectins peanut agglutinin (PNA) and Aleuria aurantia lectin (AAL) (Vector Laboratories, Inc., Newark, California, USA) in the same buffer overnight at 4◦C. ..

    Article Title: Graph attention network-driven single-cell omics analysis reveals how B4GALNT1-mediated glycosylation remodelling of the ferroptosis-pyroptosis network promotes drug resistance in hepatocellular carcinoma.
    Article Snippet: .. BIE et al. 9 of 28 Biotin-labelled lectins, including Biotin-VVA (Vector Laboratories, B-1231, USA), Biotin-SBA (Vector Laboratories, B-1015, USA), and Biotin-PNA (Vector Laboratories, B1075, USA), were added at a working concentration of 1–3 μg/mL and incubated for 1.5 h at room temperature ..

    Glycoproteomics:

    Article Title: Mucin Biology as a Local Diagnostic and Promising Therapeutic Target in Endometriosis: Expression and Glycosylation Profiling.
    Article Snippet: The primary antibodies used in this study included mouse anti-MUC1/EMA (clone E29, M0613, Agilent Technologies, Santa Clara, CA, USA), rabbit anti-MUC2 (ab134119, Abcam), mouse anti-MUC6 (sc-33668, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-MUC16/CA 125 (clone 5E1, MABC1608, Sigma-Aldrich, St. Louis, MO, USA), and mouse anti-MUC5AC (clone 45M1, MA5-12178, Thermo Fisher Scientific, Waltham, MA, USA) and rabbit antiserum MAN-5ACI for the polypeptides of MUC5AC (generous gift from Prof. Dr. David Thornton) [45]. .. In addition, several lectins were employed to detect specific glycan structures, including TRITC-labelled Dolichos biflorus agglutinin (DBA, GlycoMatrixTM, Dublin, OH, USA), biotinylated Maackia amurensis lectin II (MAL II), biotinylated Sambucus nigra lectin (SNA), FITC-labelled wheat germ agglutinin (WGA, Sigma Aldrich), biotinylated peanut agglutinin (PNA), FITC-labelled Aleuria aurantia lectin (AAL), and FITC-labelled Ulex europaeus agglutinin I (UEA I), all obtained from Vector Laboratories, Inc. (Newark, NJ, USA). ..

    Concentration Assay:

    Article Title: Graph attention network-driven single-cell omics analysis reveals how B4GALNT1-mediated glycosylation remodelling of the ferroptosis-pyroptosis network promotes drug resistance in hepatocellular carcinoma.
    Article Snippet: .. BIE et al. 9 of 28 Biotin-labelled lectins, including Biotin-VVA (Vector Laboratories, B-1231, USA), Biotin-SBA (Vector Laboratories, B-1015, USA), and Biotin-PNA (Vector Laboratories, B1075, USA), were added at a working concentration of 1–3 μg/mL and incubated for 1.5 h at room temperature ..

    Staining:

    Article Title: Generation of a new immunodeficient rat model of retinal degeneration with LSL TdTomato reporter and TdTomato-Pcp2 expression
    Article Snippet: After further washing with PBS, the slides were incubated with DAPI (4’6’-diamidino-2-phenylinode hydrochloride) for 30 min at room temperature and were coverslipped with Vectashield mounting media (Vector Labs). .. For Peanut agglutinin (PNA) lectin staining, slides were blocked with carbo-free blocking solution (CFBS; Vector labs, Cat. # CSP-5040-125) containing 3% Triton-X100 (TX), followed by biotinylated PNA (Vector labs, Cat. # B-1075-5) diluted 1:200 in CFBS without TX at 4°C overnight. .. After 3x PBS washes, slides were then incubated in DyLight 649 Streptavidin (Vector labs, Cat. # SA-5649-1; final conc.

    Blocking Assay:

    Article Title: Generation of a new immunodeficient rat model of retinal degeneration with LSL TdTomato reporter and TdTomato-Pcp2 expression
    Article Snippet: After further washing with PBS, the slides were incubated with DAPI (4’6’-diamidino-2-phenylinode hydrochloride) for 30 min at room temperature and were coverslipped with Vectashield mounting media (Vector Labs). .. For Peanut agglutinin (PNA) lectin staining, slides were blocked with carbo-free blocking solution (CFBS; Vector labs, Cat. # CSP-5040-125) containing 3% Triton-X100 (TX), followed by biotinylated PNA (Vector labs, Cat. # B-1075-5) diluted 1:200 in CFBS without TX at 4°C overnight. .. After 3x PBS washes, slides were then incubated in DyLight 649 Streptavidin (Vector labs, Cat. # SA-5649-1; final conc.



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